Injection of PGE₂ reversed the immunosuppression induced by RNA interference (RNAi) of POX-F (A) and POX-H (B).

<p>Immunosuppression was recorded as decreased nodule formation induced by injection of dsRNA, in which ‘dsPOX-F’ specific to POX-F or dsPOX-H specific to POX-H, was injected in a dose of 500 ng per larva and subsequently incubated for 48 h at 25°C. For nodulation, 5×10⁵ cells of <i>E. coli</i> were injected to each test larva and incubated for 8 h at 25°C. Aspirin, a COX-specific inhibitor, was injected in a dose of 100 ng per larva along with the bacterial challenge. Control dsRNA (‘dsCON’) was prepared against a viral gene, CpBV-ORF302. Each treatment was independently replicated three times. Different letters above error bars indicate significant difference among means at Type I error = 0.05 (LSD test).