Superoxide production from <i>L. amazonensis</i> -infected BMM at day 5 post-immune cell activation.

<p>A) BMM from wild-type C57BL/6 mice were infected with <i>L. amazonensis</i> amastigotes and co-cultured with TLN cells from <i>L. major</i> infected mice. Cultures were incubated with nitro blue tetrazolamide (NBT) for 90 minutes at the time points indicated. Cells were counterstained with eosin and viewed using light microscopy at 40x magnification. Basophilic cytoplasmic precipitate (formazan) is indicative of superoxide production within infected cells (arrows). Results are representative of three separate experiments. B) BMM from mice deficient in FcRγ-common chain were treated as in A. Cultures were incubated with NBT for 90 minutes at 5 days post-activation. C) BMM from mice deficient in gp91^phox were treated as in A and incubated with NBT at day 5 post-activation. Results in B and C are representative of two separate experiments.