Amino acid sequence alignment of G-protein subunits of <i>B. rapa</i>.

<p>(<b>A</b>) The sequence alignment of <i>B. rapa</i> (BraA.Gα1) proteins with Gα protein of rice (OsRGA1) and <i>Arabidopsis</i> (AtGPA1). Consensus sequences for GTP binding and hydrolysis are labeled with G1–G5 boxes. P/M, the predicted site for palmitoylation/myritoylation (MGXXCS); closed square, the conserved glutamine (Q222) important for the GTPase activity of Gα proteins; *, the conserved ADP ribosylation site in Gα proteins. (<b>B</b>) Amino acid sequence alignment <i>B. rapa</i> (BraA.Gβ1-3) proteins with Gβ proteins of rice (OsRGB1) and <i>Arabidopsis</i> (AtAGB1) and. Seven WD repeats are marked with horizontal bar. (<b>C</b>) Amino acid sequence alignment of <i>B. rapa</i> (BraGγ1-5) proteins with <i>Arabidopsis</i> (AtAGG1-3) proteins. Conserved DPLL motif (provides hydrophobic contact to Gβ subunit proteins), and CaaX domain (required for attachment to cell-membrane) are marked within boxes, in order. The amino-acid sequence alignment of G-protein subunits was performed using ClustalW. The conserved and divergent residues are shaded in dark and white backgrounds, respectively.