Administaration of rAg85B induced immune deviation from a Th2-response towards a Th1, Th17-related response in OVA-stimulated LN cells.

<p>OVA-immunized (i.p., day0 and 14) and sensitized (5% aerosolized-OVA, day21 to 25) BALB/c mice were challenged with PBS or rAg85B protein (i.p. (100 µg; days 0 and 14) and i.n. (20 µg; days 21, 23, and 25)). At 24 h after the last OVA sensitization, mediastinal lymph nodes (MLNs) from naïve or OVA sensitized BALB/c mice treated with PBS or rAg85B, were harvested. MLNs were cultured with OVA (50 µg/ml), and cytokines in the culture supernatant were determined 48 h after incubation by ELISA. Data are representative of at least three independent experiments (*P<0.05, **P<0.01 compared with OVA control. error bars, s.d.; n = 6 mice).