The induction of TGF-β receptors at 72 h following MCAO.

<p>Dark- and bright-field images demonstrate the induction of mRNA of the TGF-β receptors. The lesion sites are indicated by star symbols (*) and the borders of lesions are demarcated by white dots. A: TGF-β RI expression is induced in the penumbra immediately outside the lesion as well as within the infarct area. B: The mRNA of TGF-β RII is also induced in the penumbra and also within the lesion. C: TGF-β RIII mRNA expressing cells were present within the lesioned area with appearance suggesting expression in blood vessels. D: ALK1 was also seen to be induced around the vessels within the infarct area. The fields indicated by the white arrows in A–D is enlarged and shown in bright field in E–H. Cells expressing TGF-β RI (E), TGF-β RII (F), TGF-β RIII (G), and Alk1 (H) are indicated by black arrowheads. The mRNA of TGF-β RIII and Alk1 are around blood vessels. Abbreviations: cc - corpus callosum, LV - lateral ventricle. Scale bars: 1 mm for A-D, and 50 µm for E–H.