Reproductive function was improved in CTx-mice transplanted with EGFP-AFSCs.

<p>(A) The number of estrous cycles in two weeks was evaluated in the mice. Con = Non-CTx-mice (n = 5). Saline = CTx-saline-mice. (n = 5). AFSCs = CTx-AFSCs-mice. (n = 5). Data represent the mean ± S.E.M. Lower case letters (a, b, and c) represent significant differences (<i>P</i><0.05) among groups. (B) The successful mating rate was assessed in a 24-day period. The presence of a copulatory plug indicated successful mating. Con = Non-CTx-mice. (n = 10). Saline = CTx-saline-mice. (n = 10). AFSCs = CTx-AFSCs-mice. (n = 10). Log-rank (Mantel-Cox) test was used to compare the data among groups. <i>P</i><0.01 represents significant differences between groups. (C) The litter numbers were counted. Con = Non-CTx-mice (n = 10). Saline = CTx-saline-mice. (n = 4). AFSCs = CTx-AFSCs-mice (n = 10). Data represent the mean ± S.E.M. Lower case letters (a, b, and c) represent significant differences (<i>P</i><0.05) among groups. (D–F) The F1 litters showed normal gross appearance with no progeny bearing EGFP (arrow) as compared to the EGFP transgenic mice (arrowhead).