Histological Analysis of bone regeneration by implanting HBPHs with Ad-MSC.

<p>Bone defects treated with Hydrogel and Hydrogel with Ad-MSCs, 45 days after implantation. <b>a</b>, <b>b</b>, hematoxylin and eosin staining, showing a mild chronic inflammatory response. <b>c</b>, <b>d</b>, blue toluidine staining, evidencing intramembranous ossification. <b>e</b>, <b>f</b>, Masson trichrome staining, showing a better organized bone tissue and increased calcification, where hydrogels with Ad-MSC were implanted (Magnification 10×).