Differentiation capacity of TS cells <i>in vitro</i>.

<p>(a) Morphological changes of TS cells upon removal of FGF2 (-F, second from the left), activin A (-A, second from the right), or XAV939 (-X, right). FAXY represents undifferentiated TS cells as a control (left). Scale bar, 100 µm. (b) Changes in expression levels of TS cell marker genes upon removal of FGF2 (-F). (c) Changes in expression levels of TS cell marker genes upon removal of activin A (-A). (d) Changes in expression levels of TS cell marker genes upon removal of XAV939 (-X). (e) Changes in expression levels of differentiated trophoblast lineage marker genes upon removal of FGF2 (-F). (f) Changes in expression levels of differentiated trophoblast lineage marker genes upon removal of activin A (-A). (g) Changes in expression levels of differentiated trophoblast lineage marker genes upon removal of XAV939 (-X).