<p>Naïve CD4<sup>+</sup> or CD8<sup>+</sup> cells from AhR<sup>+/−</sup> mice were cultured for 96 h under Tc1/Th1 (open symbols) or Th17/Tc17 (closed symbols) polarising conditions in the presence of 3 µM CH-223191 (•,○) or 300 nM FICZ (▴,Δ) formulated in DMSO or with an equivalent amount of DMSO alone (▪,□). At selected time points (12–96 h) RNA was isolated and levels of IFN-γ (A, B), IL-17 (C, D) and IL-22 (E, F) mRNA transcripts were analysed by RT-PCR using the ΔΔ Ct method. Results were normalised against naive CD4<sup>+</sup> or CD8<sup>+</sup> cells and the housekeeping gene HPRT. A single experiment is shown as a function of relative mRNA expression on a logarithmic scale.</p
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...
<p>Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions and analyzed. A) IL-1...
<p>A, B and C are Flow cytometry analyses of Th17 development in CD4+T cells cultured in the presenc...
<p>Highly purified naive CD4<sup>+</sup> T cells from female C57BL/6 mice were incubated for 2 h wit...
<p>Naïve CD4<sup>+</sup> cells from AhR<sup>+/−</sup> (black bar) or AhR<sup>−/−</sup> mice (white b...
<p>Naïve T cells from 8-wk-old mice were stimulated under Th0, Th1, Th2, Th17, and IL-21-producing p...
<p>Naive T cells from C57BL/6 mice were cultured under Th17 polarizing conditions as described in <a...
<p>Splenocytes were harvested from C57B6 mice and bulk CD4<sup>+</sup> T cells were isolated. CD4<su...
<p>(A) Representative dot plot of IL-17 and IFN-γ production by CD4<sup><b>+</b></sup> T cells in no...
<p>CD4+CD25- T cells were stimulated in the presence of Th17 polarising cytokines for four days and ...
<p>Splenocytes from immunized mice treated with vehicle or SM934 were isolated at the peak of diseas...
<p>MACS sorted CD4<sup>+</sup>T cells from DO11.10 mice were first polarised towards a Th1 (grey bar...
<p>(A) Flow cytometric analysis of IL-17 and IL-22 production in sorted naïve mouse CD4<sup>+</sup> ...
<p>(A) Purified CD4<sup>+</sup> T were stimulated with plate-bound anti-CD3 (5 µg/ml) and titrated d...
<p>(a) CD4<sup>+</sup> T cells isolated from wild type (WT) C57BL/6 (B6) mice and IFN-γ knock out (K...
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...
<p>Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions and analyzed. A) IL-1...
<p>A, B and C are Flow cytometry analyses of Th17 development in CD4+T cells cultured in the presenc...
<p>Highly purified naive CD4<sup>+</sup> T cells from female C57BL/6 mice were incubated for 2 h wit...
<p>Naïve CD4<sup>+</sup> cells from AhR<sup>+/−</sup> (black bar) or AhR<sup>−/−</sup> mice (white b...
<p>Naïve T cells from 8-wk-old mice were stimulated under Th0, Th1, Th2, Th17, and IL-21-producing p...
<p>Naive T cells from C57BL/6 mice were cultured under Th17 polarizing conditions as described in <a...
<p>Splenocytes were harvested from C57B6 mice and bulk CD4<sup>+</sup> T cells were isolated. CD4<su...
<p>(A) Representative dot plot of IL-17 and IFN-γ production by CD4<sup><b>+</b></sup> T cells in no...
<p>CD4+CD25- T cells were stimulated in the presence of Th17 polarising cytokines for four days and ...
<p>Splenocytes from immunized mice treated with vehicle or SM934 were isolated at the peak of diseas...
<p>MACS sorted CD4<sup>+</sup>T cells from DO11.10 mice were first polarised towards a Th1 (grey bar...
<p>(A) Flow cytometric analysis of IL-17 and IL-22 production in sorted naïve mouse CD4<sup>+</sup> ...
<p>(A) Purified CD4<sup>+</sup> T were stimulated with plate-bound anti-CD3 (5 µg/ml) and titrated d...
<p>(a) CD4<sup>+</sup> T cells isolated from wild type (WT) C57BL/6 (B6) mice and IFN-γ knock out (K...
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...
<p>Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions and analyzed. A) IL-1...
<p>A, B and C are Flow cytometry analyses of Th17 development in CD4+T cells cultured in the presenc...