Kank/EB1 binding is via an EB1 binding, SxIP, motif in the middle region of Kank.

<p>(A) A summary of Kank truncations and their microtubule plus end localisation. ++ (strong localisation), + (weak localisation), - (no localisation). (B) Kank truncations which contain the middle region of Kank can co-localise with EB1 in S2 cells. S2 cells transfected with GFP-fused truncations were co-stained for GFP, EB1 and DNA. Co-localisation was observed (yellow arrows). (C) The SxIP motif in Kank was mutated to SxNK. (D) S2 cells transfected with GFP-Kank(I764N,P765K) were co-stained for GFP and EB1. Mutation of the SxIP motif in the middle region of Kank abolished co-localisation of Kank with EB1 (yellow arrows). (E) Mutation of the SxIP motif significantly decreased the percentage of cells with GFP signal localised to the majority of the observed EB1 comets. Significance was determined by Fishers exact chi squared test. Error bars show the standard error of the mean. For all microscopy images yellow boxes are areas magnified in images shown below and co-localisation is indicated by yellow arrows. Scale bars = 5 µm.