Design of Ribeye A-domain, B-domain/CtBP2s, and CtBP1 constructs.

<p>(A) Schematic representation of human and zebrafish Ribeye proteins. Sequence similarity of the two Ribeye domains between human and both zebrafish paralogs are shown as percentages. Protein alignments were performed using the ClustalW method. (B) Schematic representation of the five constructs expressed in the stable transgenic zebrafish lines used in this study. The sequence similarity between Ribeye a B-domain and CtBP1 proteins is shown as a percentage. Hair-cell specific expression of each construct was driven by the <i>myo6b</i> promoter <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107256#pone.0107256-Obholzer1" target="_blank">[11]</a>.