Comparison of IgG1-binding capacity of sera from oral sensitization studies with gluten products.

<p>Groups of rats were dosed by gavage at day 1–35 with 0.2, 2 and 20 mg/rat of unmodified gluten or hydrolysis products thereof. Sera from individual rats obtained at sacrifice were preincubated with tenfold dilutions of (50 pg/mL–50 µg/mL) of unmodified gluten (open circles), enzymatic hydrolyzed gluten (grey triangles) or acid hydrolyzed gluten (black squares) before measurement of IgG1-binding capacity by addition to ELISA plates coated with the gluten product used for sensitization. Only sera with an IgG1 titer >7 were analyzed by inhibition ELISA. Symbols represent triplicates of the same serum/inhibitor mix and bars represent IC50 median values for each group. Data were analyzed by non-parametrical analysis. Asterisks indicate statistically significant difference between groups; **: <i>p</i>≤0.01, ***: <i>p</i>≤0.001, NS: not significant.