Characterization of the DC-UbP-binding domain in USP5.

  • Ai-Xin Song (190340)
  • Hui Yang (91136)
  • Yong-Guang Gao (628147)
  • Chen-Jie Zhou (190333)
  • Yu-Hang Zhang (190330)
  • Hong-Yu Hu (170637)
Publication date
September 2014

Abstract

<p><b>A,</b> Domain architecture of USP5. NT, N-terminal domain; ZnF, zinc-finger domain; UBA1 and UBA2, tandem UBA domains (UBA12); C-box and H-box, two boxes of the USP domain. The constructs of USP5_ZnF, USP5_UBA12 and USP5_UBA1 include residues 169–289, 625–749 and 631–692, respectively. <b>B,</b> Overlay of the HSQC spectra of <sup>15</sup>N-labeled UbP_C (200 µM) and addition of UBA12 at different molar ratios. <b>C,</b> As in (B), overlay of the HSQC spectra of <sup>15</sup>N-labeled UbP_C (200 µM) and addition of ZnF at different molar ratios. <b>D,</b> Titration of UbP_C with UBA12 for measuring the binding affinity. The concentration of <sup>15</sup>N-labeled UbP_C was 150 µM. <b>E,</b> Diagram of the chemical-shift changes of UbP...

Extracted data

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