<p><b>A,</b> Domain architecture of USP5. NT, N-terminal domain; ZnF, zinc-finger domain; UBA1 and UBA2, tandem UBA domains (UBA12); C-box and H-box, two boxes of the USP domain. The constructs of USP5_ZnF, USP5_UBA12 and USP5_UBA1 include residues 169–289, 625–749 and 631–692, respectively. <b>B,</b> Overlay of the HSQC spectra of <sup>15</sup>N-labeled UbP_C (200 µM) and addition of UBA12 at different molar ratios. <b>C,</b> As in (B), overlay of the HSQC spectra of <sup>15</sup>N-labeled UbP_C (200 µM) and addition of ZnF at different molar ratios. <b>D,</b> Titration of UbP_C with UBA12 for measuring the binding affinity. The concentration of <sup>15</sup>N-labeled UbP_C was 150 µM. <b>E,</b> Diagram of the chemical-shift changes of UbP...
<p>Development of a differential scanning fluorimetry (DSF) assay for USP5 zinc finger ubiquitin bin...
<p>USP5 zinc-finger ubiquitin binding domain structure solution to 1.55 Å resolution</p
<p>The determination of binding affinities of compounds of interest against USP5 zinc finger ubiquit...
<p><b>A,</b> DC-UbP contains two separate domains, the N-terminal UBD and the C-terminal UbL. The N-...
Growth of well-diffracting co-crystals of USP5 zinc finger ubiquitin binding domain (Zf-UBD) and co...
Analysis of the solved structure of USP5 zinc finger ubiquitin binding domain (Zf-UBD) in crystalliz...
<p>Ub is colored in mauve taupe. <b>A</b>) Cartoon representation of Ub. Residues mediating interact...
<p>Determination of crystallization conditions which allow growth of well-diffracting co-crystals of...
<p>USP5 zinc-finger ubiquitin binding domain with L-cysteine(residue195)-glutathione disulfide struc...
Objective: To grow well-diffracting co-crystals of USP5 zinc finger ubiquitin binding domain (ZnF-UB...
The complicated task of interpreting the many ubiquitin signals is mediated by specific ubiquitin bi...
A surface plasmon resonance (SPR) assay was used to determine the binding affinities of USP5 zinc fi...
DC-UbP/UBTD2 is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, an...
Screening compounds against USP5 zinc finger ubiquitin binding domain (Zf-UBD) using a 19F NMR spect...
<p>HDAC6 is a unique cytoplasmic histone deacetylase characterized by two deacetylase domains, and b...
<p>Development of a differential scanning fluorimetry (DSF) assay for USP5 zinc finger ubiquitin bin...
<p>USP5 zinc-finger ubiquitin binding domain structure solution to 1.55 Å resolution</p
<p>The determination of binding affinities of compounds of interest against USP5 zinc finger ubiquit...
<p><b>A,</b> DC-UbP contains two separate domains, the N-terminal UBD and the C-terminal UbL. The N-...
Growth of well-diffracting co-crystals of USP5 zinc finger ubiquitin binding domain (Zf-UBD) and co...
Analysis of the solved structure of USP5 zinc finger ubiquitin binding domain (Zf-UBD) in crystalliz...
<p>Ub is colored in mauve taupe. <b>A</b>) Cartoon representation of Ub. Residues mediating interact...
<p>Determination of crystallization conditions which allow growth of well-diffracting co-crystals of...
<p>USP5 zinc-finger ubiquitin binding domain with L-cysteine(residue195)-glutathione disulfide struc...
Objective: To grow well-diffracting co-crystals of USP5 zinc finger ubiquitin binding domain (ZnF-UB...
The complicated task of interpreting the many ubiquitin signals is mediated by specific ubiquitin bi...
A surface plasmon resonance (SPR) assay was used to determine the binding affinities of USP5 zinc fi...
DC-UbP/UBTD2 is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, an...
Screening compounds against USP5 zinc finger ubiquitin binding domain (Zf-UBD) using a 19F NMR spect...
<p>HDAC6 is a unique cytoplasmic histone deacetylase characterized by two deacetylase domains, and b...
<p>Development of a differential scanning fluorimetry (DSF) assay for USP5 zinc finger ubiquitin bin...
<p>USP5 zinc-finger ubiquitin binding domain structure solution to 1.55 Å resolution</p
<p>The determination of binding affinities of compounds of interest against USP5 zinc finger ubiquit...