Whole-cell cryo-tomography of surface-bound rotavirus particles.

<p><b>A–C.</b> Tomographic slices through individual virions, free (<b>A</b>) and bound (<b>B,C</b>) to cell membrane. Note differences in spike extension in <b>B</b> and <b>C</b> (red arrowheads). <b>D–I.</b> Tomographic slices (<b>D–F</b>) and isosurface renderings (<b>G–I</b>) of subtomogram averages of rotavirus particles. Subtomogram averages: (<b>D,G</b> & <b>F,I</b>) after applying <i>icosahedral symmetry</i> (<b>D,G:</b> 1080 repeats from 18 virions; <b>F,I</b>: 420 repeats from 7 virions); for (<b>E,H</b>), we classified the repeats and averaged only those 78 repeats from 5 virions for which spikes were in contact with membrane (blue density).