2D analysis of PrP^C and brain- and PMCAb-derived PrP^Sc.

<p><b>A.</b> 2D analysis of Syrian hamster NBH (top panel) and NBH treated with sialidase from <i>A. ureafaciens</i> (bottom gel). <b>B, C.</b> 2D analysis of brain- and PMCAb-derived 263K material (<b>B</b>), and brain- and PMCAb-derived SSLOW material (<b>C</b>). To produce PMCAb-derived material, brain-derived 263K or SSLOW was subjected to 24 serial rounds with 10-fold dilution between rounds. Black and white triangles mark diglycosylated and monoglycosylated glycoforms, respectively, whereas arrows mark the unglycosylated form. D. Migration of PrP^C in 1D SDS-PAGE gel before and after treatment with sialidase. All blots were stained with 3F4 antibody. <b>E.</b> Schematic diagram of PrP^C that illustrates location of sialic acid residues (stars) on N-linked glycans and GPI anchor. Each of the two glycans can carry up to four terminal sialic acid residues.