Characterization of specific memory T cell subpopulations induced.

<p>Thirty days after immunization, pooled-splenocytes of six BALB/c mice were CFSE- labeled and stimulated with V₃-peptide during 4 days. Then, cells were stained with surface marker (CD8, CD44 and CD62L) and analyzed by flow cytometer. CD8 T-cells with specific proliferative capacity (evaluated by the CFSE dilution assay) were classified in four memory subpopulations. (A) Bars represent the percentage of specific CD8 T-cell with a phenotype compatible with: T stem cells memory (T_SCM; CD44L-CD62L+), T central memory (T_CM; CD44+ CD62L+), T effector memory (T_EM; CD44+ CD62L-) and T terminal effector (T_TEM; CD44- CD62L-). (B) Pie charts show the percentage of specific CD8 T-cells expressing the indicated phenotype. Statistical differences between groups are indicated: *p<0.05; ***p<0.001 by Student's T test.