Oxidation status of LptA::His_x6 in oxidoreductase mutants of <i>N. meningitidis</i>.

<p>Standardised cell lysates were separated by SDS-PAGE, followed by transfer to a membrane and western immunoblot using anti-His_x6 HRP conjugate antibody to detect the presence of LptA::His_x6. Panel A. Lane 1, protein molecular weight standard (New England Biolabs, Cat-2-212); Lane 2: NMBΔ<i>dsbA1/dsbA2</i> expressing LptA::His_x6 from pCMK1001 (CKNM221) untreated; Lane 3: CKNM221 treated with DTT and alkylated with AMS; Lane 4: CKNM221 alkylated with AMS, Lane 5: NMBΔ<i>dsbA3</i> expressing LptA::His_x6 (CKNM222) untreated; Lane 6: CKNM222 treated with DTT and alkylated with AMS; Lane 7: CKNM222 alkylated with AMS. Panel B. Lane 1, protein molecular weight standard (New England Biolabs, Cat-2-212); Lane 2: NMB expressing LptA::His_x6 (CKNM216) untreated; Lane 3: CKNM216 treated with DTT and alkylated with AMS; Lane 4: CKNM216 alkylated with AMS; Lane 5: NMBΔ<i>dsbA1</i>/<i>NmdsbA2/dsbA3</i> expressing LptA::His_x6 (CKNM755); Lane 6: CKNM755 treated with DTT and alkylated; Lane 7: CKNM755 alkylated with AMS.