CYP53A Tter and CYP53C2 Pchr 3D-models validation.

<p>Both CYP53 P450 models were based on the template CYP51 (PDB ID: 4LXJ) from <i>Saccharomyces cerevisiae</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107209#pone.0107209-Monk1" target="_blank">[42]</a> and were generated using Modeller <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107209#pone.0107209-Sali1" target="_blank">[43]</a>. Abbreviations: Tter <i>Thielavia terrestris</i>; Pchr, <i>Phanerochaete chrysosporium</i>.</p>a<p> Models were based on the template CYP51 (lanosterol 14α-demethylase) from <i>Saccharomyces cerevisiae</i> (PDB ID: 4LXJ) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107209#pone.0107209-Monk1" target="_blank">[42]</a>.</p>b<p> Sequence identity between CYP53 P450s (CYP53A Tter and CYP53C2 Pchr) and the template CYP51 (PDB ID:4LXJ).</p>c<p> Number of P450s amino acids modeled and their percentage compared to the full-length P450s.</p>d<p> dDFire and DFIRE2 pseudo-energy (lower values signify a better model) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107209#pone.0107209-Zhou1" target="_blank">[44]</a>.</p>e<p> QMEAN6 composite score ranging from 0 to 1 (higher values signify a better model) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107209#pone.0107209-Benkert1" target="_blank">[45]</a>.</p>f<p> verify3D scores ranges from -1 (bad score) to +1 (good score). This program analyzes the compatibility of an atomic model (3D) with its own amino acid sequence (1D) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107209#pone.0107209-Lthy1" target="_blank">[46]</a>.</p><p>CYP53A Tter and CYP53C2 Pchr 3D-models validation.