The xylose consumption phenotypes of the evolved Y127 and Y128 strains are dependent upon <i>CpxylA</i> and <i>ScTAL1.</i>

<p>Extracellular xylose concentrations (solid lines) and cell density (dashed lines) were measured by YSI instrument and OD₆₀₀ readings, respectively, from cultures containing KanMX marker rescued versions of (<b>A</b>) GLBRCY127 (Y132) and GLBRCY132 <i>xylAΔ</i> or (<b>B</b>) Y132 and Y132 <i>tal1Δ</i> strains inoculated in aerobic YPX media. In (<b>C</b>), extracellular xylose concentrations (solid lines) and cell density (dashed lines) were measured as in (<b>A</b>, <b>B</b>) for KanMX marker rescued GLBRCY128 (Y133) and two independent GLBRCY133 <i>xylAΔ</i> strains inoculated in anaerobic YPX media. These selection marker-rescued Y128 strains were cultured in YPD media and total RNA isolated from a single time point. Expression of <i>CpxylA</i> was then quantified and normalized to <i>ScERV25</i> RNA levels by qPCR. The bar graph in (<b>D</b>) displays the average values and standard deviations for <i>CpxylA</i> RNA from three independent biological replicates.