<p>A diagram summarizing the engineering and evolution of the YB-210 strain into the evolved Y128 strain is provided in (<b>A</b>). Fermentation kinetic profiles of the Y127 (<b>B</b>) and Y128 (<b>C</b>) strains cultured in bioreactors containing ACSH and sparged with nitrogen from biological duplicates are shown. Average concentrations and standard deviations of indicated components were quantified from media samples at times from initial inoculation. Vertical colored bars indicate time points at which samples were taken for metabolomic analysis described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107499#pone-0107499-g007" target="_blank">Fig. 7A–D</a>.</p
A) OD600 (circles), xylose concentration (squares), and ethanol concentration (triangles) for Y184 (...
Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carb...
Ethanolic fermentation is a means of utilizing xylose-rich industrial wastes, but an optimized biopr...
<p>Fermentation kinetic profiles comparing Y22-3, Y127 and Y128 strains cultured anaerobically in bi...
<p>Average fermentation kinetic profiles of the GLBRCY127 strain cultured in bioreactors containing ...
Batch cultures of Y128, Y184, Y184 hog1Δ, and Y184 Bcy1-AiD were grown anaerobically for 92 hours in...
<p>Average sugar consumption and cell growth of unevolved GLBRCY22-3 strain engineered with <i>ScTAL...
<p>Fermentation experiments were performed using 2× YNB medium containing 20 g L<sup>−1</sup> glucos...
<p>The growth was conducted in 50 mL YPXC medium containing 20 g/L xylose and 20 g/L cellobiose. The...
<p>ND, Not Determined – low xylose consumption; ND*, Not Determined – no ethanol produced and low xy...
Heterologous genes for xylose utilization were introduced into an industrial Saccharomyces cerevisi...
<p>Extracellular xylose concentrations (solid lines) and cell density (dashed lines) were measured b...
A. Average (n = 3) change in OD600 of ira2Δ, bcy1Δ, and EWY55 strains grown anaerobically in rich xy...
Abstract Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environme...
<p>DX123, D452-2 expressing one copy of <i>XYL1</i>, <i>XYL2</i>, and <i>XYL3</i>; SR6, D452-2 expre...
A) OD600 (circles), xylose concentration (squares), and ethanol concentration (triangles) for Y184 (...
Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carb...
Ethanolic fermentation is a means of utilizing xylose-rich industrial wastes, but an optimized biopr...
<p>Fermentation kinetic profiles comparing Y22-3, Y127 and Y128 strains cultured anaerobically in bi...
<p>Average fermentation kinetic profiles of the GLBRCY127 strain cultured in bioreactors containing ...
Batch cultures of Y128, Y184, Y184 hog1Δ, and Y184 Bcy1-AiD were grown anaerobically for 92 hours in...
<p>Average sugar consumption and cell growth of unevolved GLBRCY22-3 strain engineered with <i>ScTAL...
<p>Fermentation experiments were performed using 2× YNB medium containing 20 g L<sup>−1</sup> glucos...
<p>The growth was conducted in 50 mL YPXC medium containing 20 g/L xylose and 20 g/L cellobiose. The...
<p>ND, Not Determined – low xylose consumption; ND*, Not Determined – no ethanol produced and low xy...
Heterologous genes for xylose utilization were introduced into an industrial Saccharomyces cerevisi...
<p>Extracellular xylose concentrations (solid lines) and cell density (dashed lines) were measured b...
A. Average (n = 3) change in OD600 of ira2Δ, bcy1Δ, and EWY55 strains grown anaerobically in rich xy...
Abstract Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environme...
<p>DX123, D452-2 expressing one copy of <i>XYL1</i>, <i>XYL2</i>, and <i>XYL3</i>; SR6, D452-2 expre...
A) OD600 (circles), xylose concentration (squares), and ethanol concentration (triangles) for Y184 (...
Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carb...
Ethanolic fermentation is a means of utilizing xylose-rich industrial wastes, but an optimized biopr...