MP production is increased in hepatic IRI.

<p><b>A</b>. MP release (concentration expressed as phosphatidylserine, PS equivalent in nM) and <b>Bi,ii</b> serum ALT and hyaluronic acid (HA) after 60 min ischemia and indicated reperfusion times in naïve and Diannexin-treated mice (n = 10 per cohort). * p<0.05 all experimental groups vs. sham. ‡ p<0.05 IRI 2 hr vs. 30 min reperfusion. # p<0.05 IRI 24 hr vs. 15 min, 30 min and 2 hr reperfusion. § p<0.05 Diannexin vs. no Diannexin at 24 hr reperfusion. <b>C</b>. FACS plots demonstrating MPs released early in reperfusion are composed predominantly of endothelial-derived/CD144 remnants while in late reperfusion, MPs are largely from leukocytes and hepatocytes. (<b>Ci</b>) FACS data depicted as % bearing cell-specific markers (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104376#pone.0104376.s003" target="_blank">Table S1</a>). Note, because individual MPs are cell fragments and may potentially fuse, MPs can bear more than 1 cell-marker and therefore, the sum of all expressed markers may feasibly exceed 100% (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104376#pone.0104376.s004" target="_blank">Materials and Methods S1</a>). (<b>Cii</b>) Annexin V was utilized to analyze for double positive events together with cell-specific markers CD144, CD41, CD62P F4/80 and Ly6G. * p<0.05 IRI groups vs. sham. <b>D</b>. MPs bear ASPGR (hepatocytes) and VE-cadherin (SECs), VCAM-1, ICAM-1, E-selectin, P-selectin. MPs obtained from mice subjected to liver post-IR at indicated times. Blots representative of three similar experiments. <b>E</b>. Image analyses/quantification show upregulation of adhesion molecules especially at 2, 24 hr reperfusion. * p<0.05 IRI vs. sham. & p<0.05 IRI 2 and 24 hr vs. IRI 15 and 30 min reperfusion. %p<0.05 IRI 30 min vs. IRI 15 min reperfusion. # p<0.05 IRI 24 hr vs. 15,30 min, 2 hr reperfusion. § p<0.05 IRI 2 hr vs. 15 min reperfusion. ‡ p<0.05 IRI 2 hr vs. IRI 30 min reperfusion. <b>F</b>. MPs at 15 min and 2 hr reperfusion contain increased levels of F2-isoprostanes compared to those derived from sham-operated mice by GCMS and LC/MS/MS (normalised to total AA detected).