Effects of DZNep and 5AZA on let-7b and EZH2 levels.

<p><b>A</b>) DZNep exposure at different concentrations (0.5 µM and 1 µM) significantly reduced let-7b expression in KG1a, MDS-L and P-L21 cells (p = 0.005 and p = 0.002, respectively). The results are displayed as mean ±SEM of 3 similar experiments (Student t-test). <b>B</b>) KG1a cells treated with DZNep showed decreased cells in S phase. <b>C</b>) Treatment of KG1a cells, <b>D</b>) healthy donor cells, and <b>E</b>) cells from MDS marrow, with DZNep, 5AZA or a combination of both, decreased let-7b expression, more so with the combination than with 5AZA alone (p = 0.049 and p = 0.002, respectively, results show the mean±SEM of 3 experiments; Student's t-test, U6B served as loading control)); <b>F</b>) MDS-L cells showed decreased H3K27m2 and H3K27m3, and decreased EZH2 protein expression.; Histone H3 served as nuclear loading control. The blots show one of 3 similar experiments.