Effect of HU on IGF-1 level.

<p>(A) IGF-1 level in cerebral tissues, determined by ELISA, in control (C) and HU14 groups. (B) IGF-1 measurement in plasma. B1: Circulating IGF-1 determined by ELISA. B2: Total IGFBP3 (left) and IGFBP5 (right) expressed with respect to total protein level. B3: Quantification of IGF-1 bound to IGFBP3 (left) or IGFBP5 (right), expressed as the ratio between bounded IGF-1 and total IGFBPs, and representative western blot showing IGFBPs after IGF-1 immunoprecipitation. Box and whisker plots show the median (horizontal bar), quartiles (box), and extreme values (whiskers). *** p<0.001 with respect to C value.