Inhibition of TNF-α-induced IL-6 expression by 5-LOX inhibitors in human synovial fibroblasts.

<p>Human synovial fibroblasts were incubated with TNF-α (10 ng/ml) for indicated time intervals. mRNA and released IL-6 were determined by QPCR and ELISA respectively. Treatment with TNF-α enhanced the IL-6 mRNA (A) expression and cytokine release (B) in a time-dependent manner. Pretreatment with two 5-LOX inhibitors, NDGA (5 or 10 µM) or MK-886 (5 µM) for 1 hr significantly inhibited the TNF-α-induced mRNA (C) or protein levels (D) of IL-6. Data are presented as mean ± SEM. *, p<0.05 compared with vehicle control (con). #, p<0.05 compared with TNF-α treatment alone.