<p>Human synovial fibroblasts were pretreated with NDGA (10 µM) or MK-886 (5 µM) for 1 hr, and then treated with TNF-α for another 6 hr. Cell lysate was extracted for real-time PCR evaluation (A) and conditioned medium was collected for ELISA measurement of MCP-1 (B). Note that 5-LOX inhibitors NDGA and MK-886 antagonized TNF-α-induced mRNA and protein levels of MCP-1/CCL-2. Data are presented as mean ± SEM. *, p<0.05 compared with control (con). #, p<0.05 compared with TNF-α-treatment alone.</p
<p>(A, B and C) HepG2-X (or H7402-X) cells were treated with PDTC (0, 20, 40 and 60 µM) for 6 h. (A)...
<p>Immunofluorescent staining showed that p65 translocated into nucleus after treatment of TNF-α (25...
ABSTRACT Proinflammatory cytokines, altered connective tissue metabolism, and overexpression of matr...
<p>Human synovial fibroblasts were incubated with TNF-α (10 ng/ml) for indicated time intervals. mRN...
<p>(A) Immunoblotting showed that treatment of TNF-α (10 ng/ml) enhanced the phosphorylation of IκBα...
<p>Total protein extracts from RASFs transfected with empty vector or 5-LOX shRNA were used for West...
<p>TNF-α increased the release of IL-6 and MCP-1 via the activation of NF-κB signaling in RASFs. 5-L...
<p>Fibroblast-like synoviocytes were treated with AVX002 (2 hrs) in indicated concentrations (A, B),...
The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human rheumato...
<div><p>The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human ...
<p>Human synovial fibroblasts were pretreated with LTB4 receptor antagonist LY29311 (1 µM) for 1 hr,...
The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human rheumato...
The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human rheumato...
OBJECTIVE: To investigate the role of the transcription factor nuclear factor-kB (NF-kappaB) in prom...
<p>Soluble chimeric monoclonal anti-TNF-α antibody (infliximab) was added to the culture supernatant...
<p>(A, B and C) HepG2-X (or H7402-X) cells were treated with PDTC (0, 20, 40 and 60 µM) for 6 h. (A)...
<p>Immunofluorescent staining showed that p65 translocated into nucleus after treatment of TNF-α (25...
ABSTRACT Proinflammatory cytokines, altered connective tissue metabolism, and overexpression of matr...
<p>Human synovial fibroblasts were incubated with TNF-α (10 ng/ml) for indicated time intervals. mRN...
<p>(A) Immunoblotting showed that treatment of TNF-α (10 ng/ml) enhanced the phosphorylation of IκBα...
<p>Total protein extracts from RASFs transfected with empty vector or 5-LOX shRNA were used for West...
<p>TNF-α increased the release of IL-6 and MCP-1 via the activation of NF-κB signaling in RASFs. 5-L...
<p>Fibroblast-like synoviocytes were treated with AVX002 (2 hrs) in indicated concentrations (A, B),...
The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human rheumato...
<div><p>The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human ...
<p>Human synovial fibroblasts were pretreated with LTB4 receptor antagonist LY29311 (1 µM) for 1 hr,...
The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human rheumato...
The lipoxygenase isoform of 5-lipoxygenase (5-LOX) is reported to be overexpressed in human rheumato...
OBJECTIVE: To investigate the role of the transcription factor nuclear factor-kB (NF-kappaB) in prom...
<p>Soluble chimeric monoclonal anti-TNF-α antibody (infliximab) was added to the culture supernatant...
<p>(A, B and C) HepG2-X (or H7402-X) cells were treated with PDTC (0, 20, 40 and 60 µM) for 6 h. (A)...
<p>Immunofluorescent staining showed that p65 translocated into nucleus after treatment of TNF-α (25...
ABSTRACT Proinflammatory cytokines, altered connective tissue metabolism, and overexpression of matr...