Relationship between cytotoxic activities of gas phase extracts of cigarette smoke and cigarette brand.

<p>The gas phase extracts of cigarette smoke (designated cCSE) at the original tar concentration of 10 mg/ml were prepared from cigarettes of various brands by continuous smoking protocol as described in the legend for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107856#pone-0107856-g001" target="_blank">Fig. 1</a>. The cCSEs were subjected to MTS reduction assay for evaluation of their cytotoxic activities, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107856#pone-0107856-g003" target="_blank">Fig. 3</a>. MTS reduction activity in the absence of the gas phase extract was represented as 100%. Values represent means ± SE of three experiments, each in triplicate. P, Peace (JT, Japan; 28 mg tar, 2.3 mg nicotine), HL, Hi-Lite (JT, Japan; 17 mg tar, 1.4 mg nicotine), SS, Seven Stars (JT, Japan; 14 mg tar, 1.2 mg nicotine), M, Mevius (JT, Japan; 10 mg tar, 0.8 mg nicotine), MSL, Mevius Super Light (JT, Japan; 6 mg tar, 0.5 mg nicotine), Ma, Marlboro (Phillip Morris, USA; 12 mg tar, 1.0 mg nicotine), LS, Lucky Strike (British American Tobacco, UK; 11 mg tar, 0.9 mg nicotine), K9, Kent 9 mg (British American Tobacco, UK; 9 mg tar, 0.8 mg nicotine).