Fz1-MO and Stbm-MO have very similar effects on gene expression profiles.

<p>Each set of 3 panels shows typical <i>in situ</i> hybridization patterns obtained for uninjected embryos (left), Fz1-MO embryos (center) and Stbm-MO embryos (right) injected, fixed at the early gastrula stage and processed in parallel. A-B: O-type pattern transcripts (Myb and ZnfO); C-D: IE-type pattern transcripts (FoxA and Znf845); E-F: A-type pattern transcripts (ZnfA and sFRP-A); G-H: D-type pattern transcripts (Botch1 and bZip). Cells with high Botch1 and bZip expression on the blastocoelar face of the ectoderm were discernible (narrows) in Fz1MO and StbmMO embryos. Gene identity is shown in the bottom left of each set of panels. For each transcript the two morpholinos have very similar effects on the expression patterns, confirming the z-score comparisons (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004590#pgen.1004590.s001" target="_blank">File S1</a>). Scale bar 50 µm.