In pancreas, epiplakin colocalizes with ductular and acinar keratins and its apicolateral localization in acinar cells is dependent on keratin filaments.

<p>(A–L) Immunofluorescence microscopy for epiplakin, K7, K8 and K19 in pancreata of wild-type mice. Nuclear staining is shown for easier distinction of ductal and acinar cells. Epiplakin is expressed in ductal cells (dc) and in the apicolateral compartment (al) of acinar cells (B, F), where it colocalizes with K8 and K19 (C, G). K7 signals were found in ductal cells but were absent from acinar cells (J). (M–P) Immunofluorescence microscopy for epiplakin and K8 in pancreata of K8-deficient (K8^−/−) mice. In K8^−/− pancreata, epiplakin is expressed in ductal cells but is absent from acinar cells (N). Whole acini are outlined by dashed lines for orientation. Asterisks indicate cytosolic/perinuclear areas. Scale bars, 20 µm.