Monocyte subsets display distinct gene expression.

<p>CD14⁺CD16⁻, CD14⁺CD16⁺ and CD14^loCD16⁺ monocytes from healthy donors and <i>P. vivax</i>-infected patients were isolated by FACS. (A) Representative dot plots showing monocyte subpopulations before (left panel) and after FACS-sorting (right panels). (B) A nanostring analysis of monocyte subsets from 5 patients infected with <i>P. vivax</i> and 5 healthy donors. Heatmap representation of 41 differentially regulated genes upon malaria infection compared to healthy donors is depicted. (C) <i>CCL2</i>, <i>CXCL2</i>, <i>ICAM1</i>, <i>NLRP3</i>, <i>TNFR1/TNFRSF1A</i>, <i>NFKB1</i>, <i>REL</i>, <i>NFKB1A</i>, <i>IFNGR2</i>, <i>IL1RA</i>, <i>IL8</i>, <i>CASP1</i>, <i>CR1</i>, <i>CD80</i>, <i>IL10</i>, <i>IL6</i> and <i>TNF</i> were differentially induced among CD14⁺CD16⁻ (green bars), CD14⁺CD16⁺ (red bars) and CD14^loCD16⁺ (blue bars) monocytes from <i>P. vivax</i>-infected patients. *<i>p</i><0.05, **0.05><i>p</i>>0.01, ***<i>p</i><0.01.