Positive and negative NES datasets obtained from four different data resources.

<p>(<b>A</b>) Artificial NES datasets. (<b>B</b>) DUB NES datasets. (<b>C</b>) Valid NES datasets. (<b>D</b>) Sp-protein datasets. The positive and negative datasets (B-P2 and B-N2) of the DUB datasets and the negative training dataset (D-N2) of the Sp-protein datasets were always included in the training data for the profile optimization, whereas the other training datasets were used only when they were not contained in a test dataset to be used. For example, when we conducted the prediction test with the test datasets, A-P1 and A-N1, we used the optimized profiles for NESmapper, that were trained with C-N2, in addition to B-P2, B-N2, and D-N2.