TLR7-mediated γδ T cell activation during WNV infection.

<p>Splenic T cells of wild type, MyD88^−/− and TLR7^−/− mice were isolated before infection (day 0) and at days 3 and 5 post-infection and stained for TCRγδ, Vγ1⁺, Vγ4⁺ and CD3. ** <i>P</i><0.01 or * <i>P</i><0.05 compared to wild-type mice. <i>A</i>. Total γδ⁺ percentage among splenic T cells. <i>B</i>. Splenic γδ cell number per mouse. <i>C–D</i>. Vγ1⁺ (<i>C</i>) or Vγ4⁺ (<i>D</i>) percentage among splenic T cells. <i>E</i>–<i>G</i>. Wild-type B6 mice were injected with R848 followed by infection with WNV NS4B-P38G mutant. Cells were harvested at day 3 post-infection and stained for TCRγδ, Vγ1⁺, Vγ4⁺ and CD3. <i>E</i>. Total γδ⁺ percentage among splenic T cells. <i>F</i>. Splenic γδ cell number per mouse. <i>G</i>. Total number of Vγ1⁺ and Vγ4⁺ cells per mouse. ** <i>P</i><0.01 or * <i>P</i><0.05 compared to control group.