The effects of TLR2, 4 and 7 agonists on anti-CD3- treated murine γδ T cells.

<p><i>A</i>, Flow cytometry analysis of splenic γδ T cells stained with antibodies to TCRγδ and CD3. <i>B–E</i>, splenic γδ T cells were cultured with anti-CD3 with or without TLR agonists. Cells were harvested at 48 h post-stimulation and examined for CD69 expression (<i>B</i>), and IFN-γ (<i>C</i>), IL-2 (<i>D</i>) and TNF-α (<i>E</i>) production in culture supernatant. <i>F</i>. <i>In vitro</i> T cell proliferation assay. CFSE- labeled γδ⁺ T cells were cultured for 48 h in the presence of anti-CD3 with or without TLR agonists. Data shown are fold of increase of T cell proliferation compared to anti-CD3 treated cells. <i>G</i>. CD25 expression. Data are presented as means ± SEM, <i>n</i> = 4–7. ** <i>P</i><0.01 or * <i>P</i><0.05 compared to anti-CD3- treated cells. Data presented are one representative of at least four similar experiments.