Ectopic expression of ICP22 or the 193–256 subdomain causes loss of pol II CTD Ser2 phosphorylation.

<p>Alignment of the conserved core motif within ICP22 of the alphaherpesviruses showing the degree of conservation according to the scheme above. Alignments were generated using PRALINE (<a href="http://www.ibi.vu.nl/programs/pralinewww/" target="_blank">http://www.ibi.vu.nl/programs/pralinewww/</a>). Asterisks indicate identical residues. Accession Nos. AEDO2597, AEV91400, AAA46092, CAA54262, AEL30878 (<b>B</b>) Top, diagram of full-length ICP22 and 193–256 showing the position of the alphaherpesvirus conserved core motif and the epiptope tags (Myc). Bottom, the results of western blot analysis of extracts from HeLa cells transfected with the constructs indicated using antibodies to the Myc tag. α-tubulin was used as a loading control. (<b>C</b>) Western blot analysis of whole-cell extract from HeLa cells transfected with the constructs indicated using antibodies to the Ser2P CTD phosphorylation mark. RPAP2 was used as a loading control.