The <i>lprG</i> mutant is impaired for macrophages entry.

<p>(A) Resting BMMΦ from C57BL/6 mice were infected with wild-type (H37Rv), <i>lprG</i> mutant (Δ<i>lprG</i>), and Δ<i>lprG</i> complemented with <i>lprG</i>-Rv1410c (::<i>lprG</i>) at MOI of 1∶10 (Input). At 4 h post-infection cells were washed and intracellular bacteria (Output) were enumerated on agar. Bar graph shows average CFU counts ±SEM of three independent experiments performed in triplicate wells. (B–D) RAW 264.7 macrophages treated with and without 4 mg/ml mannan or 0.5 µg/ml anti-MMR pAb were infected with FITC-labeled bacteria at MOI of 1∶100 for 2 h. Cells were washed, mounted and visualized using a fluorescent microscope. Graphs show percentage of infected cells with no treatment (B), treated with mannan (C) and treated with anti-MMR pAb (D). Bars show average percentage ±SEM of two independent experiments performed in triplicates. Δ<i>lprG</i> and ::<i>lprG</i> were compared to H37Rv. *P<0.05.