The effect of ROCK inhibition on the acquisition of an EMT phenotype.

<p>Western blot analysis showed that MHCC97H cells subjected to Matrigel with culture medium treated (<b>C</b>) or not treated (<b>A</b>) with Y27632 for 48 h regulated Vimentin and E-cadherin expression. GAPDH was used as a loading control. Quantification of EMT markers is shown in (<b>B</b>) and (<b>D</b>). The data are reported as mean ± S.E. (*<i>P</i><0.05 compared to 0 h or the control, n = 3).