ROCK inhibition decreases the motility of MHCC97H cells.

<p>(<b>A</b>) Y27632 inhibition delayed wound healing. Representative images of scratch wounds formed at 0, 24 and 48 h after wounding. Original magnification was 100×, scale bars represent 125 µm. (<b>C</b>) Y27632 inhibition decreased invasiveness. Invaded cells attached at the lower surface of the filters were stained with crystal violet. Original magnification was 200×, scale bars represent 75 µm. (<b>B</b>) and (<b>D</b>) Data were normalized to untreated cells and the relative migration or invasion is expressed as mean ± S.E. of triplicate experiments. Fewer cells migrated or invaded 48 h after incubation with Y27632 (50 µM), compared with the control cells, *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001.