TUNEL apoptosis detection(200×magnification).

<p>(A). Extensive TUNEL-positive cells, including osteocytes, chondrocytes and bone-marrow cells were observed in the femoral heads of the model group. (B). In the prevention group, relatively lower numbers of apoptotic cells were observed. (C). Bar graph shows the comparison of apoptotic rate between the model group and the prevention group. In the prevention group, the apoptotic rate was significantly lower than that in the model group(P<0.05). The asterisk (*) shows statistically significant differences between the two groups. The arrows indicated TUNEL-positive cells.