UDP stimulated translocation of NF-κB.

<p>The 16HBE14o- cells were stimulated with vehicle control (A, B) and 100 µM UDP (C, D) for 60 min. The nuclei (A, C) were stained with Hoechst 33342, and NF-<i>κ</i>B (B, D) was stained with anti-NF-<i>κ</i>B and Alexa Fluor 488 goat anti-rabbit secondary antibody (magnification 20×). (E) Immunofluorescence of NF-<i>κ</i>B in the nuclear region and cytoplasmic region was quantified using NIH ImageJ software. Nucleus∶cytoplasm ratios of NF-κB staining were calculated. Each column represents the mean ± S.E. (<i>n</i> = 3) (*<i>p</i><0.01 compared with vehicle control, Student’s <i>t</i>-test).