Fluid accumulation studies.

<p><b>A</b>. This is the graphical representation of fluid accumulation ratio in infant mice model. For fluid accumulation studies 5 days old infant mice were infected with 100 µl of 1×10⁶ CFU/ml of wild type <i>V. cholerae</i>, Δ<i>chiA2</i> mutant, and the complemented strain. Mice were sacrificed after 3, 6, 12 and 18 hours. The fluid accumulation calculated and presented graphically. In all the above three experiments PBS was used as negative control and the data expressed as means ± SEM of three independent experiments (P<0.01). <b>B</b>. Rabbit ileal loop response of wild type <i>V. cholerae</i> cells (1×10⁹ CFU/ml) showing hemorrhagic fluid accumulation whereas, infection with Δ<i>chiA2</i> mutant (1×10⁹ CFU/ml) shows significant decrease in fluid accumulation. Infection with the complemented strain showed similar hemorrhagic fluid accumulation like the wild type strain. PBS was used as negative control. The experiment was repeated thrice (n = 3). <b>C</b>. This is the graphical representation of fluid accumulation ratio in rabbit ileal loop. The fluid accumulation ratio for the Δ<i>chiA2</i> mutant (FA/cm ratio: 0.04±0.01) was 40-fold less compared to the wild type <i>V. cholerae</i> (FA/cm ratio: 1.6±0.2). The fluid accumulation ratio for the complemented strain was similar with the wild type. PBS was used as negative control (FA/cm ratio: 0.02±0.008). The experiment was repeated thrice (n = 3) and the data expressed as means ± SEM.