Utilization of mucin as nutrient for growth by <i>V. cholerae</i>.

<p><i>V. cholerae</i> wild type, Δ<i>chiA2</i> mutant and the complemented strain were separately grown in LB upto log phase. The log phase cultures of the wild type <i>V. cholerae</i>, Δ<i>chiA2</i> mutant and the complemented strain were centrifuged and washed in PBS. The minimal media supplemented with 2% (w/v) porcine mucin and fresh LB broth were inoculated separately with equal number of washed <i>V. cholerae</i> wild type and Δ<i>chiA2</i> mutant and complemented strain. The number of bacteria survived with increasing incubation time was detected by plate count method for all of the strains. The viable counts of bacteria were graphically represented. <b>A</b>. Viable count when mucin used as nutrient. <b>B</b>. Viable count when grown in LB broth. Each of the experiment was repeated thrice (n = 3) and the data expressed as means ± SEM.