Role of ChiA2 in <i>V. cholerae in vitro</i> and <i>in vivo</i> proliferation.

<p><b>A</b>. Comparative study of the survival of wild type and Δ<i>chiA2</i> mutant in human intestinal HT29 cell line. The graph represents a dose dependent survival of wild type <i>V. cholerae</i> and Δ<i>chiA2</i> mutant and also a complemented strain. <b>B</b>. Comparative study of dose dependent mice intestinal survival of wild type <i>V. cholerae</i>, Δ<i>chiA2</i> mutantand the complemented strain. For the experiment, 5 days old suckling mice were fed with different dilutions of the mentioned strains and sacrificed after 16 hours. The viability of the strains was measured by cell count method. Bars: (□) PBS; (▪) wild type <i>V. cholerae</i>; ( ) Δ<i>chiA2</i> mutant () complemented strain. Each of the experiment was repeated thrice (n = 3) and the data expressed as means ± SEM.