<p>(A) Maximal increase of cytosolic Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) of <i>Efhd2<sup>+/+</sup></i> (black bars) and <i>Efhd2<sup>-/-</sup></i> platelets (gray bars) after activation with the indicated agonists (thrombin 0.1 U/ml, CRP 4 µg/ml). (B) Flow cytometric analysis of phosphatidylserine (PS) exposure in response to the indicated agonists in <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> platelets. Washed platelets were stained with Annexin-V-DyLight-488 in the presence of Tyrodes-HEPES buffer containing 3 mmol/L Ca<sup>2+</sup>. The values represent the mean fluorescence intensity (MFI) ± SD for 5 mice per group in 3 independent experiments. Thr: thrombin, CRP: collagen related peptide, CVX: c...
With a combined phase-contrast and fluorescence video imaging system, changes in morphology and cyto...
<p>A) WT and GRIP1<sup>-/-</sup> platelets have similar agonist-induced activation. Washed platelets...
<p>(A) MPs from human platelets (stimulated with collagen) labeled with the CMFDA cell tracker were ...
<p>Flow cytometric analysis of integrin αIIbβ3 activation (A) and degranulation-dependent P-selectin...
1 U/ml thrombin, 10 μM ADP, or 10 μg/ml CRP in the presence of extracellular 1 mM EGTA or 0.5 mM Ca,...
<p>(A) Analysis of EFhd2 expression in <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> pla...
<p>(A) Generation of ROS from platelets is reported as mean fluorescence intensity (MFI) of DCF. Bar...
<p>Fura 2-loaded wild type (black line) and PAR3<sup>−/−</sup> (gray line) platelets were resuspende...
<p>(A) Flow cytometric analysis of integrin αIIbβ3 activation (upper panel) and degranulation-depend...
<p>A,B,C. Platelet aggregation measured in platelet rich plasma (1 x 10<sup>8</sup> platelets/assay)...
<p>(A) Platelets become activated by stimulation with 50 ng A<i>ß</i> (1–40) as shown by TEM (restin...
<p>(A-C) Washed platelets of <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> mice were all...
<p>Wild-type and <i>Myo5a</i><sup>−/−</sup> platelets were loaded with the Ca<sup>2+</sup>-sensitive...
<p>(A) Analysis of EFhd1, Rac1, mDia1, actin and tubulin expression in <i>Efhd2<sup>+/+</sup></i> an...
<p>Washed platelets from P2Y<sub>12</sub> deficient mice were labeled with 12.5 µM Fura-2/AM/0.2% Pl...
With a combined phase-contrast and fluorescence video imaging system, changes in morphology and cyto...
<p>A) WT and GRIP1<sup>-/-</sup> platelets have similar agonist-induced activation. Washed platelets...
<p>(A) MPs from human platelets (stimulated with collagen) labeled with the CMFDA cell tracker were ...
<p>Flow cytometric analysis of integrin αIIbβ3 activation (A) and degranulation-dependent P-selectin...
1 U/ml thrombin, 10 μM ADP, or 10 μg/ml CRP in the presence of extracellular 1 mM EGTA or 0.5 mM Ca,...
<p>(A) Analysis of EFhd2 expression in <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> pla...
<p>(A) Generation of ROS from platelets is reported as mean fluorescence intensity (MFI) of DCF. Bar...
<p>Fura 2-loaded wild type (black line) and PAR3<sup>−/−</sup> (gray line) platelets were resuspende...
<p>(A) Flow cytometric analysis of integrin αIIbβ3 activation (upper panel) and degranulation-depend...
<p>A,B,C. Platelet aggregation measured in platelet rich plasma (1 x 10<sup>8</sup> platelets/assay)...
<p>(A) Platelets become activated by stimulation with 50 ng A<i>ß</i> (1–40) as shown by TEM (restin...
<p>(A-C) Washed platelets of <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> mice were all...
<p>Wild-type and <i>Myo5a</i><sup>−/−</sup> platelets were loaded with the Ca<sup>2+</sup>-sensitive...
<p>(A) Analysis of EFhd1, Rac1, mDia1, actin and tubulin expression in <i>Efhd2<sup>+/+</sup></i> an...
<p>Washed platelets from P2Y<sub>12</sub> deficient mice were labeled with 12.5 µM Fura-2/AM/0.2% Pl...
With a combined phase-contrast and fluorescence video imaging system, changes in morphology and cyto...
<p>A) WT and GRIP1<sup>-/-</sup> platelets have similar agonist-induced activation. Washed platelets...
<p>(A) MPs from human platelets (stimulated with collagen) labeled with the CMFDA cell tracker were ...