EFhd2 is dispensable for platelet production.

<p>(A) Analysis of EFhd2 expression in <i>Efhd2^+/+</i> and <i>Efhd2^-/-</i> platelets by Western blot. Expression of GPIIIa was used as loading control. (B) Peripheral platelet counts and (C) platelet volume of <i>Efhd2^+/+</i> and <i>Efhd2^-/-</i> mice measured with a blood cell counter are depicted. Results are mean ± SD of 7 mice per group. (D) Determination of the platelet life span in wild-type and <i>Efhd2^-/-</i> mice. Mice were injected with a DyLight 488-conjugated anti-GPIX derivate (0.5 µg/g body weight) to label platelets <i>in vivo</i>. Results are% of fluorescently labeled platelets at the indicated days after injection as determined by flow cytometry. Values are mean ± SD of 5 mice per group. (E) Determination of MK numbers per visual field (294×221 µm) in hematoxylin and eosin stained BM sections. Values are mean ± SD (n = 5).