Normal integrin outside-in signaling in <i>Efhd2^-/-</i> platelets.

<p>(A-C) Washed platelets of <i>Efhd2^+/+</i> and <i>Efhd2^-/-</i> mice were allowed to spread on fibrinogen (100 µg/mL) for 30 min after stimulation with 0.01 U/mL thrombin. (A) Statistical evaluation of the percentage of spread platelets at different spreading stages and (B) representative differential interference contrast (DIC) images of 2 individual experiments. 1: roundish, 2: only filopodia, 3: filopodia and lamellipodia, 4: fully spread. (C) Visualization of filamentous actin (red) in spread (30 min) <i>Efhd2^+/+</i> and <i>Efhd2^-/-</i> platelets by cofocal microscopy. (D) Clot retraction of prp upon activation with 4 U/mL thrombin in the presence of 20 mmol/L CaCl₂ at the indicated time points (n = 6).