<p>Flow cytometric analysis of integrin αIIbβ3 activation (A) and degranulation-dependent P-selectin exposure (B) in response to the indicated agonists in <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> platelets. Results are mean fluorescence intensities (MFI) ± SD of 4 mice per group and are representative of 4 individual experiments. CRP: collagen-related peptide, CVX: convulxin, and RC: rhodocytin.</p
<p>The results are representative of four separate experiments. Platelets from the following mice we...
<p>(<b>A</b>) Detection by western blot of α2 integrin in equivalent total protein samples from mono...
<p>Wild-type and <i>Myo5a</i><sup>−/−</sup> mouse platelets were stimulated for 10 min with the indi...
<p>(A) Flow cytometric analysis of integrin αIIbβ3 activation (upper panel) and degranulation-depend...
<p>(A) Maximal increase of cytosolic Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) o...
<p>(A-C) Washed platelets of <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> mice were all...
<p>(<b>A</b>) Dose-response curves for activation of integrin α<sub>IIb</sub>β<sub>3</sub> in respon...
<p><i>A</i>: Washed platelets from WT or KO mice were stimulated with CRP (1 or 5 µg/ml) in the pres...
<p>(A) Analysis of EFhd2 expression in <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> pla...
<p>Washed platelets from WT (black lines) and <i>Pdlim7</i><sup>-/-</sup> (red lines) mice were stim...
1 U/ml thrombin, 10 μM ADP, or 10 μg/ml CRP in the presence of extracellular 1 mM EGTA or 0.5 mM Ca,...
Testing of platelet function is central to the cardiovascular phenotyping of genetically modified mi...
<p>Washed platelets from <i>Efhd2<sup>+/+</sup></i> (black line) and <i>Efhd2<sup>-/-</sup></i> (gra...
<p>(A) Aggregation and ATP secretion. Washed platelets from PKCε-deficient (PKCε<sup>−/−</sup>) mice...
<p>(A) Hic-5, leupaxin and paxillin expression was assessed by Western blot analysis. GAPDH served a...
<p>The results are representative of four separate experiments. Platelets from the following mice we...
<p>(<b>A</b>) Detection by western blot of α2 integrin in equivalent total protein samples from mono...
<p>Wild-type and <i>Myo5a</i><sup>−/−</sup> mouse platelets were stimulated for 10 min with the indi...
<p>(A) Flow cytometric analysis of integrin αIIbβ3 activation (upper panel) and degranulation-depend...
<p>(A) Maximal increase of cytosolic Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) o...
<p>(A-C) Washed platelets of <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> mice were all...
<p>(<b>A</b>) Dose-response curves for activation of integrin α<sub>IIb</sub>β<sub>3</sub> in respon...
<p><i>A</i>: Washed platelets from WT or KO mice were stimulated with CRP (1 or 5 µg/ml) in the pres...
<p>(A) Analysis of EFhd2 expression in <i>Efhd2<sup>+/+</sup></i> and <i>Efhd2<sup>-/-</sup></i> pla...
<p>Washed platelets from WT (black lines) and <i>Pdlim7</i><sup>-/-</sup> (red lines) mice were stim...
1 U/ml thrombin, 10 μM ADP, or 10 μg/ml CRP in the presence of extracellular 1 mM EGTA or 0.5 mM Ca,...
Testing of platelet function is central to the cardiovascular phenotyping of genetically modified mi...
<p>Washed platelets from <i>Efhd2<sup>+/+</sup></i> (black line) and <i>Efhd2<sup>-/-</sup></i> (gra...
<p>(A) Aggregation and ATP secretion. Washed platelets from PKCε-deficient (PKCε<sup>−/−</sup>) mice...
<p>(A) Hic-5, leupaxin and paxillin expression was assessed by Western blot analysis. GAPDH served a...
<p>The results are representative of four separate experiments. Platelets from the following mice we...
<p>(<b>A</b>) Detection by western blot of α2 integrin in equivalent total protein samples from mono...
<p>Wild-type and <i>Myo5a</i><sup>−/−</sup> mouse platelets were stimulated for 10 min with the indi...