H₂S-Nampt pathway regulates bioenergetics.

<p>(A) Amount of H₂S released from DR^{H W1} HepG2 cells treated with CTH inhibitor, PAG (100 µM, 18 hr), and Nampt inhibitor, FK866 (200 nM, 24 hr). (B) Western blot analysis of CBS in DR cells in the absence and presence of FK866 (200 nM, 24 hr). (C) Western blot analysis of CTH in DR cells in the absence and presence of FK866 (200 nM, 24 hr). (D) ATP levels in DR^{H2O2 W3} HepG2 cells in the absence (−) and presence (+) of PAG (100 µM, 18 hr). (E) ATP levels in Pc, DR^{H2O2 W2} and DR^{H2O2 W3} HepG2 cells in the absence and presence of FK866 (200 nM, 24 hr). (F) Western blot analysis of Nampt in DR^{H2O2 W2} and DR^{H2O2 W3} HepG2 cells treated with CBS inhibitor, CHH (500 µM, 18 hr) or CTH inhibitor, PAG (100 µM, 18 hr). *; <i>p</i><0.05,**; <i>p</i><0.005, ***; <i>p</i><0.0005.