Oxidation state of Trx1 and Trx2 in different strains defective in maintaining redox homeostasis.

<p>Wild-type and mutated cells were processed for western blotting as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108123#s4" target="_blank">Material and Methods</a>. Proteins were detected with rabbit polyclonal anti-Trx2 (A, C) and anti-Trx1 (B) antibodies. Extracts from wild-type cells, treated with 0.5 mM H₂O₂ for 5 min in YPG, was loaded as a control (B, lane 7).