BrdU incorporation increases during MA.

<p>(<b>A–I</b>) Immunofluorescence labeling for GS (red) (<b>A, B, C</b>), BrdU (green) (<b>D, E, F</b>) and respective colocalization between both (<b>G, H, I</b>), in a L5 DRG of a control and a 7d MA animal (bar represents 100 µm). <b>A</b>rrows point to well visible double-labeled SGCs. An amplified image from a L5 DRG of a 7d MA animal shows BrdU labeling in detail (bar represents 20 µm) (<b>C, F, I</b>). (<b>J</b>) The number of proliferating SGCs (SGCs⁺), in the total number of neuronal profiles (<b>SGC⁺_total/NP_total</b>), significantly increases at 7d MA. (<b>K</b>) The mean number of proliferating SGCs around a specific neuron (<b>Mean SGC⁺/NP</b>) also increases at 7d MA. (<b>L</b>) The number of positive neuronal profiles (<b>NP⁺_total/NP_total</b>) is also significantly higher in 7d MA, when compared with both control non-inflamed and to 4d MA animals. All values shown as Mean±SEM. N = 6 for controls and 7d MA, and N = 5 for 4d MA experimental group.* Significant differences relatively to control. # Significant differences relatively to 4d MA.* or ^# represents p<0.05; ** represents p<0.01. One-way ANOVA was followed by Newman-Keuls Multiple Comparison post-hoc test.