Effect of neonatal G-MDSC on T cell proliferation.

<p>(<b>A</b>) Proliferative responses of purified T cells in the presence or absence of G-MDSC after anti-CD3/CD28 bead stimulation (n = 28 independent experiments performed in duplicate for CD3 plots, n = 6 for CD4 and CD8 plots). Significance determined by the Wilcoxon Matched-Pair Signed Rank test. (<b>B</b>) Proliferative responses of purified adult naïve T cells (n = 9 independent experiments performed in duplicate) compared to cord blood T cells (n = 28 independent experiments performed in duplicate) after anti-CD3/CD28 bead stimulation. Statistical significance determined by the Mann Whitney test. (<b>C</b>) Suppression of T cell proliferation by autologous G-MDSC titration. (n = 4 independent experiments performed in duplicate). (<b>D</b>) Suppression of T cell proliferative responses by G-MDSC is contact dependent. (n = 5 independent experiments performed in duplicate). (<b>E</b>) G-MDSC frequency correlates with suppression of T cell proliferation by G-MDSC. G-MDSC frequencies were correlated to suppression of T cell proliferation by G-MDSC using the Spearman rank correlation test (n = 16 independent experiments).