Minimal levels of γ-secretase components are found in SV fractions.

<p>Analysis of <b>γ</b>-secretase subunits distribution in brain fractions isolated from WT mice. SV, P43 and SP fractions were prepared using the protocol described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108576#pone-0108576-g001" target="_blank">Fig. 1</a> and analyzed for the presence of the <b>γ</b>-secretase components Ps-1, Ps-2, Nct and Pen2 using Western blots. While all four γ-secretase components were readily detected in the P43 and, albeit at lower levels, SP fractions, only Ps-1 was detected, at very minimal level, in the SV fraction. Gapdh was used to normalize the loading.